| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229471 |
| Chromosome: | chromosome 2 |
| Location: | 5633558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109366 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTAGACTGCTAGCCCCCTAGCTCTGAA |
| Internal bar code: | GCACTCAAGAGCTCCGCATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 98.8671 |
| LEAP-Seq n confirming: | 7505 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 265 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGAAGCGAAGAAGGTC |
| Suggested primer 2: | CAACGAGTAGGTGACTGCCA |