| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229499 |
| Chromosome: | chromosome 7 |
| Location: | 5581149 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351750 | (1 of 1) K14823 - rRNA-processing protein EBP2 (EBP2, EBNA1BP2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTTATCCTTTCTTTGCCCTCCGTGAC |
| Internal bar code: | CCCCGCCTGGCAGAGCACGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 451 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCAGTATCCCATTCTTC |
| Suggested primer 2: | GAGACTGTTTGCTTGCCCTC |