| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229524 |
| Chromosome: | chromosome 9 |
| Location: | 6842558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409801 | (1 of 2) PTHR11373:SF4 - DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGCATCAGCAGGTGCGGCGGGGGTG |
| Internal bar code: | TTTGCACGCGCGTCTTCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.4264 |
| LEAP-Seq n confirming: | 5027 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAACTCCCCCTGCAGTC |
| Suggested primer 2: | ATCAAAGATCCCGCTGTCAC |