| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229543 |
| Chromosome: | chromosome 7 |
| Location: | 3379351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTAATGGCTCCGCCCCTCTGTGTGTA |
| Internal bar code: | GGGAATCGACGAGTGGAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 96.614 |
| LEAP-Seq n confirming: | 428 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCCGTAGCGCCAGATACC |
| Suggested primer 2: | GGAAGGATGTTGTTGCTGGT |