| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229545 |
| Chromosome: | chromosome 3 |
| Location: | 4188835 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173950 | (1 of 3) PF08495 - FIST N domain (FIST) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACCCTAACGCTAACACGTCTGCCGCCT |
| Internal bar code: | AAGTCTAGTCTATTGATGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.1629 |
| LEAP-Seq n confirming: | 43475 |
| LEAP-Seq n nonconfirming: | 367 |
| LEAP-Seq n unique pos: | 352 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGAAATCACACAGGCATA |
| Suggested primer 2: | ACACACAGTGAGCTGCGAAC |