| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229588 |
| Chromosome: | chromosome 14 |
| Location: | 1385669 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617300 | PSL3 | (1 of 1) PTHR12174//PTHR12174:SF42 - SIGNAL PEPTIDE PEPTIDASE // SUBFAMILY NOT NAMED; Signal peptide peptidase-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACGCATCGTTTAGCTGCACCCATTGCG |
| Internal bar code: | CGGCCCGTGCGGATCCGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 81.3084 |
| LEAP-Seq n confirming: | 2175 |
| LEAP-Seq n nonconfirming: | 500 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGATATCATGGACCGT |
| Suggested primer 2: | CATGACTTGGGTTCGAAGGT |