| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229591 |
| Chromosome: | chromosome 3 |
| Location: | 2501690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159800 | (1 of 1) PTHR21262//PTHR21262:SF0 - GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASE // GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGACGCGGGTGTGGCGCGTCAGCGGGCG |
| Internal bar code: | TCGAGAGCGAGGGTCCCACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.2785 |
| LEAP-Seq n confirming: | 13759 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCCTAGGTAGTGGTGGC |
| Suggested primer 2: | CTCACAGCGCATGGAATCTA |