| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229626 |
| Chromosome: | chromosome 10 |
| Location: | 1754728 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430700 | FAP308 | Flagellar Associated Protein 308 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAAACGTGCCACCTGGGAGTCGTTGTGC |
| Internal bar code: | GCGCGGCAGGTTTGCCCAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 78.8638 |
| LEAP-Seq n confirming: | 8593 |
| LEAP-Seq n nonconfirming: | 2303 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTTTGCTTGTGTTGTTG |
| Suggested primer 2: | CTCGTGTCCCCTTCGTATGT |