| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229631 |
| Chromosome: | chromosome 17 |
| Location: | 1187810 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACTGCCCTCCGGAAATCGCTCACCTCA |
| Internal bar code: | TCTACTCCGACACGCTCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.6574 |
| LEAP-Seq n confirming: | 2036 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCTGGTACAAGAGGACA |
| Suggested primer 2: | CCCTCTGTAACATCCGCATT |