Insertion junction: LMJ.RY0402.229636_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.229636
Chromosome:chromosome 13
Location:3742090
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAGCCGCGGCGCGGGGCTTGACCTTGGT
Internal bar code:TTTATCGTCTCCAGCGAAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:951
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACTCGCTTTCACTCCA
Suggested primer 2:GAGTGGCGCTACAGTGACAA