| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229638 |
| Chromosome: | chromosome 2 |
| Location: | 5066382 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104725 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTCGGATGTCCCGTTCGGCCGGACAGT |
| Internal bar code: | GTGTCTTCTAGTTTTGCTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 99.4526 |
| LEAP-Seq n confirming: | 1090 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCGTGAGGAGCTTGATT |
| Suggested primer 2: | ACAGGGTACGAGACGCAATC |