| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229654 |
| Chromosome: | chromosome 4 |
| Location: | 3681744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGTCTACTACGCTGGCACTGCACACG |
| Internal bar code: | ATGAGAGCTGGTGTGTTAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 99.7302 |
| LEAP-Seq n confirming: | 2218 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGTATGGGAGCGACGAGA |
| Suggested primer 2: | TATTGCTGCTGTGGTGCTTC |