| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229660 |
| Chromosome: | chromosome 16 |
| Location: | 6860535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676197 | RPN1 | (1 of 1) K03028 - 26S proteasome regulatory subunit N1 (PSMD2, RPN1); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTATACTGCTCATATGCGGCCTTGGTC |
| Internal bar code: | GTGCCCCCGCCTTACGGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 94.3925 |
| LEAP-Seq n confirming: | 202 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGTGGAAGACGGTATG |
| Suggested primer 2: | GGAGGAGGAGTGGTGTGTGT |