| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229681 |
| Chromosome: | chromosome 10 |
| Location: | 3824213 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTTGCACTAGATGGGTTTGGGATGGGT |
| Internal bar code: | AGGTCTGCGATCCAGCTTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 88.6364 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTCATCTGCTCAGCTCC |
| Suggested primer 2: | CCCCACATACATTCTGTCCC |