| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229690 |
| Chromosome: | chromosome_14 |
| Location: | 2732361 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g626800 | OPR64 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGGCGTTCGTTGGAGCCTGGCAAGTTGC |
| Internal bar code: | ATGACGACTGTAATTGGCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 98.3165 |
| LEAP-Seq n confirming: | 3212 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTACGTCCATCACCCAGC |
| Suggested primer 2: | TACTACCCACTGCTCACCCC |