Insertion junction: LMJ.RY0402.229690_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.229690
Chromosome:chromosome 16
Location:396830
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g693500 FAP40,ISG1,ISG-C1 Hydroxyproline-rich Flagellar Associated Protein 40; (1 of 5) PTHR16631:SF9 - GLUCAN 1,3-BETA-GLUCOSIDASE 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACTGAAGCCTAAGTACCCGCCCGCCAA
Internal bar code:TCAACCCGGTTTCCCCAACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:46.6667
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGCTCTCCACAACATCA
Suggested primer 2:GGGTACCATCATGCTATGGG