Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.229706 |
Chromosome: | chromosome_6 |
Location: | 7275026 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g298500 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCTGCCCTGAAGAGCAACCTCAATGTGCGT |
Internal bar code: | TTGCAATTTTGAGTGGTAGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1062 |
LEAP-Seq percent confirming: | 96.0609 |
LEAP-Seq n confirming: | 17534 |
LEAP-Seq n nonconfirming: | 719 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCAGTCTAGTCGAGGGAAG |
Suggested primer 2: | TTCTGCTTCATGATTGCTGG |