Insertion junction: LMJ.RY0402.229725_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.229725
Chromosome:chromosome 14
Location:3908649
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632847 ELG44 Exostosin-like glycosyltransferase 44; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCTCTCCCCACCCCCTGCAGGACTCG
Internal bar code:AGGCCCACGGGGTCGAGACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:386
LEAP-Seq percent confirming:98.6784
LEAP-Seq n confirming:448
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACCGGACATACCCTCCT
Suggested primer 2:CAAATCCTCGTACTCCCTGC