| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229726 |
| Chromosome: | chromosome 6 |
| Location: | 2990351 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273413 | (1 of 1) K14835 - ribosomal RNA methyltransferase Nop2 [EC:2.1.1.-] (NOP2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTAATTATGGGGGTGGGGCGGCGAAGAC |
| Internal bar code: | TGAAATTACTTAGTTGGTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.682 |
| LEAP-Seq n confirming: | 627 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGCAACAACCAAGCCC |
| Suggested primer 2: | CACAAGCTGTTGGGAGAACA |