Insertion junction: LMJ.RY0402.229739_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.229739
Chromosome:chromosome 8
Location:1996086
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAACACACACACACACACACCTTCAACT
Internal bar code:AGAGGTGGACTAGTCGTTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:255
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACGGCTTCTATGTGGTGGT
Suggested primer 2:AGAGATTGGGTGACGGAATG