| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229749 |
| Chromosome: | chromosome 13 |
| Location: | 3769398 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589650 | (1 of 1) PF08424 - NRDE-2, necessary for RNA interference (NRDE-2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCTACCACTACGGTACTTCCCTGACC |
| Internal bar code: | CCCGGTGGAACAGGCCGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 99.0338 |
| LEAP-Seq n confirming: | 205 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAGAATTAGTCAACGCA |
| Suggested primer 2: | CAGTAGCATCGGCAGTAGCA |