| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229752 |
| Chromosome: | chromosome 2 |
| Location: | 7189143 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146300 | (1 of 2) PTHR10027 - CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGAATCTCATCCTTTCTTCATCCTCGC |
| Internal bar code: | GTCGCCCGCTCCGACTGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 99.7204 |
| LEAP-Seq n confirming: | 1070 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGCGGGTACTCTGATAA |
| Suggested primer 2: | GTCTGGTGAGCAAAAGAGCC |