| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229769 |
| Chromosome: | chromosome_17 |
| Location: | 3201715 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g722150 | PKS3 | Type III polyketide synthase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCATCCAGATGGAGGAAGGCGGATCAGGA |
| Internal bar code: | ATTTGGGTACATCTCGATGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 690 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | AACCACCACAACAAGAAGGG |