| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229775 |
| Chromosome: | chromosome 2 |
| Location: | 2525244 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092400 | (1 of 1) PTHR10693 - RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTATTTGTATTCCCAGCCTGCGCCCGAC |
| Internal bar code: | AGGGCCCGGAGAGTACTCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 99.8002 |
| LEAP-Seq n confirming: | 2498 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGACTATCGCTAACGCGG |
| Suggested primer 2: | AGCTTCTTCAGCTCTGCCTG |