Insertion junction: LMJ.RY0402.229813_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.229813
Chromosome:chromosome 12
Location:3214417
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g498800 (1 of 1) PTHR23176:SF0 - RHO GTPASE-ACTIVATING PROTEIN 68F 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATGACAAATATTGATCAACGTAAAAATA
Internal bar code:AACCTCTGAATCGCGTGTCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:543
LEAP-Seq percent confirming:98.2759
LEAP-Seq n confirming:399
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGGAGATGGACACATGC
Suggested primer 2:CAAACTTCGACGGACATCCT