| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229817 |
| Chromosome: | chromosome 13 |
| Location: | 3322289 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586550 | CYB5-4,CYB4 | Cytochrome b5 flavohemoprotein; (1 of 1) PTHR19359//PTHR19359:SF10 - CYTOCHROME B5 // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGACGAAAAAGCCGCGCGAGTTCGGCG |
| Internal bar code: | CGTTGTGCACACGAGAGACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATCATGTTTCGTTGCTT |
| Suggested primer 2: | ATGCTGGAACAAACCACACA |