| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229819 |
| Chromosome: | chromosome 9 |
| Location: | 6441598 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407110 | (1 of 1) PTHR12461:SF16 - 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGGTTGCTTTGTGAGCGTACGACTGT |
| Internal bar code: | TCGGGTTCCCGAGATTCGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.092 |
| LEAP-Seq n confirming: | 17680 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAGTTCTTTCGTCTCCTG |
| Suggested primer 2: | TGCACCCTACGGGACTTTAC |