Insertion junction: LMJ.RY0402.229819_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.229819
Chromosome:chromosome 13
Location:1716188
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g574350 (1 of 4) IPR000719//IPR001245//IPR002290//IPR006189//IPR011009//IPR020635 - Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // CHASE domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGCGGCAGGTTCTACAGTGTCTGGGCAG
Internal bar code:TAGGGTCGCGTGCCACATGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:549
LEAP-Seq percent confirming:93.1775
LEAP-Seq n confirming:1953
LEAP-Seq n nonconfirming:143
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGCCATGTATTCATTTG
Suggested primer 2:GCAATAAATCCCACCCACAC