| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229832 |
| Chromosome: | chromosome_6 |
| Location: | 6499717 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g293100 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTCAGCCCACACAGCTGCCGTCAGCTCCC |
| Internal bar code: | GGGTGGAGGTCCTTGGCAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 98.6434 |
| LEAP-Seq n confirming: | 509 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTAGCTCAGTCAAGGTG |
| Suggested primer 2: | GCACATGTGAGACTGTCGCT |