| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229880 |
| Chromosome: | chromosome 10 |
| Location: | 3349172 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443850 | (1 of 1) PTHR11851//PTHR11851:SF136 - METALLOPROTEASE // PROTEIN YHJJ | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGTTAACCATCTTACCAGCAACACCAGC |
| Internal bar code: | GGGGACAGCGGGGCCCAAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTACCCTACCTCGCACA |
| Suggested primer 2: | CGACTTCCCATGCTTAGCTC |