| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229901 |
| Chromosome: | chromosome 11 |
| Location: | 1425770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467726 | (1 of 1) K06316 - oligosaccharide translocation protein RFT1 (RFT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTTTGAGGCCCGGCTGCCTTCTGCACG |
| Internal bar code: | ATGGGACCATGCACGCTGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAGCACCCTCACTTCA |
| Suggested primer 2: | CCTGACGAGGTTGGAAAAGA |