| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229923 |
| Chromosome: | chromosome 13 |
| Location: | 2088586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577450 | GPA3 | (1 of 1) 1.1.5.3 - Glycerol-3-phosphate dehydrogenase / sn-glycerol-3-phosphate dehydrogenase; Glycerol-3-phosphate acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGACTACCTGGACATCCAGGTGACTG |
| Internal bar code: | AGTCGCTCACTAGCTCGGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 96.9911 |
| LEAP-Seq n confirming: | 548 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTCAGACCAGGTGAGG |
| Suggested primer 2: | CGCTTAAGGGCAAGAGACTG |