| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229932 |
| Chromosome: | chromosome_14 |
| Location: | 1044203 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g614550 | CLR7,MDB1,OPR62 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGTAAGATGGATGCGCAACGGGTTGGGGC |
| Internal bar code: | GTCAGAGGCTCTATGTTTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 99.1724 |
| LEAP-Seq n confirming: | 4194 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATGAGCTGTGTAGACGA |
| Suggested primer 2: | GATGATGATGATGAGGGCG |