Insertion junction: LMJ.RY0402.229976_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.229976
Chromosome:chromosome 6
Location:2678544
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g270200 PLC1,PLC Phospholipase C; (1 of 1) 3.1.4.11 - Phosphoinositide phospholipase C / Triphosphoinositide phosphodiesterase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACACGTGTTCCGTCTCCATCCAGCAACT
Internal bar code:TCGCGTATAATTGTGGTGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:452
LEAP-Seq percent confirming:99.0724
LEAP-Seq n confirming:2243
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCAAATGAATGGTGTTG
Suggested primer 2:AGGGCAACAATTACACAGGC