| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229990 |
| Chromosome: | chromosome 17 |
| Location: | 6087269 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741350 | KIN7-4,KIN7D | Kinesin motor protein; (1 of 4) K11498 - centromeric protein E (CENPE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCGTCATCCATTCCTTTTACGGCGTTT |
| Internal bar code: | GCGGGTGTGAGTGTGGGTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 99.2188 |
| LEAP-Seq n confirming: | 2413 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCACTGCATTGATGCCT |
| Suggested primer 2: | GTAGCCTGGTATGCAACGGT |