Insertion junction: LMJ.RY0402.230003_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.230003
Chromosome:chromosome 17
Location:3159094
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g721750 ELG9 Exostosin-like glycosyltransferase 9; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAAGACCCAGAGTTTTAGGGACAAGGGC
Internal bar code:ATCGTAGTGATCATAACCAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:99.7797
LEAP-Seq n confirming:453
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGAAGGAGCTGTTGTCC
Suggested primer 2:ACACTCCTACCAGGGTCACG