Insertion junction: LMJ.RY0402.230033_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.230033
Chromosome:chromosome 9
Location:794860
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g402051 CLV4 Voltage-gated chloride channel; (1 of 1) PTHR11689//PTHR11689:SF89 - CHLORIDE CHANNEL // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCCCGCATCCCCACAGGCTCCCTCGCT
Internal bar code:CTGGTCCCCCACGATCTGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:263
LEAP-Seq percent confirming:59.7426
LEAP-Seq n confirming:325
LEAP-Seq n nonconfirming:219
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCCCTCCATCCTACCACC
Suggested primer 2:ATGAGGTTGTGGAGGTGGAG