Insertion junction: LMJ.RY0402.230051_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230051
Chromosome:chromosome 8
Location:249685
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g358554 (1 of 14) 3.1.3.16//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTGGATGTGTCACGTGCGGCCCACCTGC
Internal bar code:TTGTGATCGGGGAAAGATCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:94.1667
LEAP-Seq n confirming:113
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCCCTGTAAGACGAAAC
Suggested primer 2:GGAGCCAGACCAGGACATAA