Insertion junction: LMJ.RY0402.230061_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230061
Chromosome:chromosome 5
Location:1078282
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g246100 (1 of 1) IPR019887 - Transcription regulator AsnC-type, C-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGAGTGCGTGCACACTCGTCAAATAAC
Internal bar code:TTTCCCGGGACGCCGTACATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:39
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTAGCTGCCAGAGCATTC
Suggested primer 2:GGTTCTCAACTGCTTCAGGC