| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230068 |
| Chromosome: | chromosome 7 |
| Location: | 3708637 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337650 | PDC1 | Mitochondrial pyruvate dehydrogenase complex, E1 component, alpha subunit; (1 of 1) PTHR11516//PTHR11516:SF40 - PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR // PDHA1 PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGGCCGTTGGCCAGCGCGTAGGCCTT |
| Internal bar code: | TCGCGTGCCCGGGAGGAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 99.4785 |
| LEAP-Seq n confirming: | 1526 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTATGTGCGTGTGCATGT |
| Suggested primer 2: | CACGTGTGCATCACCATGTA |