Insertion junction: LMJ.RY0402.230094_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.230094
Chromosome:chromosome_13
Location:4608875
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GGTAAAGTGTATTCCAGTACACCGCAGCAA
Internal bar code:TTACTTCTCGGTGTCGCTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:99.2891
LEAP-Seq n confirming:2095
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATACCTGTACGCGCTTTGA
Suggested primer 2:CCAGCCCAAACTAAACCAAA