Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.230094 |
Chromosome: | chromosome_13 |
Location: | 4608875 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g604050 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTAAAGTGTATTCCAGTACACCGCAGCAA |
Internal bar code: | TTACTTCTCGGTGTCGCTGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 491 |
LEAP-Seq percent confirming: | 99.2891 |
LEAP-Seq n confirming: | 2095 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATACCTGTACGCGCTTTGA |
Suggested primer 2: | CCAGCCCAAACTAAACCAAA |