| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230097 |
| Chromosome: | chromosome 8 |
| Location: | 2697688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373300 | MAPKKK1 | (1 of 1) K04417 - mitogen-activated protein kinase kinase kinase 9 [EC:2.7.11.25] (MAP3K9, MLK1); Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGTCAAGCCCGGTGCGGCCACAGACG |
| Internal bar code: | GCCGCCCCGACGGAGCGCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 98.5577 |
| LEAP-Seq n confirming: | 820 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCTCATCCACCAGTGCT |
| Suggested primer 2: | CGTCCAGCTGTCAGTTTTGA |