| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230113 |
| Chromosome: | chromosome_16 |
| Location: | 2252643 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g658900 | TRP10 | Transient receptor potential ion channel protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCAAAGCAAGCATAGTATTGTGCCCGACA |
| Internal bar code: | AGCCGGGGCCACCCCGATTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.1004 |
| LEAP-Seq n confirming: | 661 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGAGTTACACCGCTTG |
| Suggested primer 2: | TCGCTGTCTTTCATGTCCTG |