| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230120 |
| Chromosome: | chromosome 12 |
| Location: | 4284322 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519550 | OPR53 | OctotricoPeptide Repeat protein 53 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGGCGGGTTGGGCTGGGCCAGGCGCA |
| Internal bar code: | TACCATCGGTACGTGAATATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCAACCACGTATTGTCC |
| Suggested primer 2: | ACAGCTCAGAAGCTGAAGGC |