| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230133 |
| Chromosome: | chromosome 12 |
| Location: | 4464114 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521550 | (1 of 1) PF00630//PF02493 - Filamin/ABP280 repeat (Filamin) // MORN repeat (MORN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGTAAGACTTCCGGTCCGGTCCGCCCA |
| Internal bar code: | GGGGAGCGCGCCGAGGAATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 75.2 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTACAGCAACAGCAGC |
| Suggested primer 2: | TGAGTTGCTAGTGGTGCTGG |