Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.230137 |
Chromosome: | chromosome 1 |
Location: | 4898268 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g033950 | EXN2 | 3'-5' exonuclease with GRF zinc finger domain; (1 of 1) K18417 - ERI1 exoribonuclease 2 [EC:3.1.-.-] (ERI2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTTGCAGCGTCGCCATGAGCCGCGCCC |
Internal bar code: | ATCGTTTAGGGGCAGAGCGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 312 |
LEAP-Seq percent confirming: | 98.4772 |
LEAP-Seq n confirming: | 388 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTCTACACAGCTCATCCG |
Suggested primer 2: | CGGCACCAAAGACCTGTAAT |