| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230137 |
| Chromosome: | chromosome 16 |
| Location: | 966823 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648750 | (1 of 1) PF10373 - Est1 DNA/RNA binding domain (EST1_DNA_bind) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCGCCGCCGCCGCCGGACTGAAGCGG |
| Internal bar code: | TTATTTTTGCGAACTATATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.4456 |
| LEAP-Seq n confirming: | 15247 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCGCTTATGATGGTCG |
| Suggested primer 2: | TAGCAGTAACCACGGGCTTC |