| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230141 |
| Chromosome: | chromosome 1 |
| Location: | 6357332 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046652 | (1 of 3) K01866 - tyrosyl-tRNA synthetase (YARS, tyrS); Putative tyrosyl tRNA synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAATGGGGATGTTACAGAGGGTCCTTAA |
| Internal bar code: | ATTATCAAGCGTGAAGGACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 96.337 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGTGTGTTGTCATCCTG |
| Suggested primer 2: | GAGGAGATGCAGGTACGGAG |