Insertion junction: LMJ.RY0402.230153_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.230153
Chromosome:chromosome 10
Location:1472869
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g428692 (1 of 1) IPR000945//IPR002110//IPR020683 - Dopamine beta-hydroxylase-related // Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGGCGGGCAGCCCGTGTTGGCTTGGCT
Internal bar code:TTGGTGAATGGAGTACGGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:434
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAACGACAGGGATGTTGG
Suggested primer 2:GAGGTAGCTCTCGAACACGG