| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230171 |
| Chromosome: | chromosome 2 |
| Location: | 5049280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104650 | (1 of 1) K14573 - nucleolar protein 4 (NOP4, RBM28) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGCACGAGTCGGCGCATGAGCAGCCA |
| Internal bar code: | ATTCGGTGTGTGCAGCGTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.2266 |
| LEAP-Seq n confirming: | 1283 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCGTAAAGTAAGCGAGA |
| Suggested primer 2: | TGTTGTGTCGATTATGCCGT |